Year 10 Science · Unit 1 · Lesson 9

Gene Editing and CRISPR

Foundation Worksheet

Name
Date
Class

Learning Goals

Match each term to its definition

Draw a line connecting each term on the left to its correct definition on the right. Or write the matching letter next to each term.

TermYour answerDefinition
CRISPR-Cas9A. Changes DNA in sperm, eggs or embryos, changes can be inherited by future generations.
Guide RNAB. An unintended DNA change at a location other than the intended cut site.
Gene driveC. A short RNA molecule designed to match a specific DNA target sequence, directing Cas9 to the correct location.
Germline editingD. A gene-editing tool using a guide molecule to find a DNA sequence and an enzyme to cut it at that exact site.
Somatic editingE. A genetic system engineered to spread a trait through a wild population at a faster-than-normal rate.
Off-target effectF. Changes DNA in body cells only, affects the individual but cannot be passed to offspring.

True or False? Fix the false ones

Circle T or F for each statement. If the statement is false, rewrite it correctly on the line below.

CRISPR-Cas9 was developed from scratch by scientists in a laboratory in the 1990s.

Correct it:

T
F

Germline editing changes that are made to an embryo can be inherited by the edited person's future children.

Correct it:

T
F

In 2023 the first CRISPR-based medicine was approved for treating sickle cell disease and beta-thalassemia.

Correct it:

T
F

CRISPR is less precise than older gene editing methods because it uses a protein enzyme rather than RNA to find the target.

Correct it:

T
F

1. In simple terms, describe the roles of the guide RNA and the Cas9 protein in the CRISPR gene editing process.

Recall 2 marks

2. He Jiankui, a Chinese scientist, edited human embryos in 2018 and was sentenced to prison. State one specific reason why the international scientific community condemned his actions.

Recall 2 marks

Wrap Up

In one sentence, what makes CRISPR different from earlier methods of genetic modification?